DIY Bio

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DIYbio or Do-It-Yourself Biology, is the undertaking of the life sciences outside of formal institutional settings. This subreddit is a place to share resources and discuss all things relating to DIYbio.

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Does anyone have thoughts on how to characterize binding affinity of designed binders on the cheap? I was thinking maybe florescently labeled condensates could be used by checking the condensation transition and comparing it to known binders, but this conflates a lot of variables together so I'm not sure if that's a workable idea.

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I found the discussion about using minimal media and sugars as a selection mechanism to be particularly interesting.